mass spec core
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For protein quantitation applications, learn more about tandem mass tags, SILAC metabolic labeling systems, SureQuant targeted Mass Spec Assays or HeavyPeptide AQUA Custom Synthesis Service. Figure 2. Techniques such as difference gel electrophoresis (DIGE), isobaric tag for relative and absolute quantitation (iTRAQ) and 18O-labeling as well as non-labeling methods (MudPit, multi-dimensional protein identification technology) are available for quantifying relative differences in protein expression and post-translational modifications.
Figure 1. Skip to the content of this page, Notice of Privacy Practices(Patients & Health Plan Members). The instrument is a Q Exactive HF-X with high and low flow UHPLCs as well as CE for the interfaces. The EasyPep Mini MS Sample Prep Kit provides a high peptide yield, and comparable peptide and protein identifications in cell lysates with good reproducibility. Routine mass spec services include molecular weight determination, MS^n, LC-MS, high resolution MS, protein identification, PTMs analysis, nano LC-MS/MS and database search, quantitative analysis of small molecule/protein, lilpidomics, metabolomics, proteomics and more.
Non-redundant protein group identification numbers are reported for each sample type. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Total recovered desalted peptides were estimated using the Pierce Quantitative Colorimetric Peptide Assay. (A) Sensitivity of Pierce Quantitative Colorimetric Peptide Assay compared to the Pierce Micro BCA Assay using BSA Digest. Thermo Fisher Scientific. Mass Spectrometry Core Beyond making available state-of-the-art, user-friendly facilities and services, the MSC laboratory enables education, methods development, and new applications development, designed to meet the fast-pace needs of researchers. We offer high quality reagents and complete, convenient kits that have been fully tested to enable optimal outcomes, from protein digestion to peptide enrichment, clean-up, and quantitation. Create Account, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Protein Quantitation Using Mass Spectrometry, Calibration Solutions, Standards, and Solvents for Mass Spectrometry, Plasma Protein Binding Equilibrium Dialysis, Thermo Scientific Tandem Mass Tag Research Awards, High-Select HSA/ Immunoglobulin Depletion Resin (A36368), High-Select Top 14 Abundant Protein Depletion Resin (A36372), Pierce Trypsin Protease, MS Grade (90057), Trypsin/Lys-C Protease Mix, MS-Grade (A41007), High Select Fe-NTA Phosphopeptide Enrichment Kit (A32992), Pierce High pH Reversed-Phase Fractionation Kit (84868), Pierce Detergent Removal Spin Columns (87777), Colorimetric Peptide Quantitation Assay (23275), Fluorometric Peptide Quantitation Assay (23290), Pierce Peptide Retention Time Calibration Mixture (88321), Pierce BSA Protein Digest Standard (88341), Pierce TMT11plex Yeast Digest Standard (A40939), Pierce Trifluoroacetic Acid (TFA) (28904), HeavyPeptide AQUA Custom Synthesis Service, Pierce Quantitative Colorimetric Peptide Assay, Mass Spectrometry Reagents Support Center. Greater than 95% cleavage selectivity for lysine and arginine (K/R) was observed for Pierce Trypsin Protease. General Information | Self-Checker | Donate and Lend Support | Staff Appreciation | Get Email Alerts. The Mass Spectrometry Facility located within the Wine Research Centre at the University of British Columbia offers a comprehensive range of research services for the food and beverage industry including analyses of fragrance and aroma compounds, flavonoids and anthocyanin’s in fruits, as well as metabolite profiling of small molecules. Research Areas: mass spectrometry, proteomics, cancer. We continue to monitor COVID-19 cases in our area and providers will notify you if there are scheduling changes. In order to minimize COVID-19 spread, the Research Resources Center, Mass Spectrometry Core is implementing the following temporary mandatory guidelines for using this core: 1737 West Polk St., Suite 310 AOB, Chicago, IL60612, © 2020 The Board of Trustees of the University of Illinois, Bioanalytics, Biophysics and Cytomics Division, Bioanalytics, Biophysics & Cytomics Division, Scientific Imaging & Nanotechnology Division, Research Histology and Tissue Imaging Core, All customers entering the core space must wear new, clean gloves, If there is a need to remove gloves, wash your hands with soap frequently and for at least 20 seconds. Don't have an account ? Figure 4.
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